caa a22 4500 465753701 CHVBK 20180323111841.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01625731 doi (NATIONALLICENCE)springer-10.1007/BF01625731 Localization of endogenous osteocalcin in neonatal rat bone and its absence in articular cartilage: Effect of warfarin treatment [Elektronische Daten] [Georges Boivin, Gérard Morel, Jane Lian, Chantal Anthoine-Terrier, Paul Dubois, Pierre Meunier] Summary: Immunocytochemistry after cryoultramicrotomy was used to localize endogenous osteocalcin in bone (calvaria, femoral diaphysis) and epiphyseal femoral cartilage from 8-day-old rats treated (or mot) for 7 days with warfarin. Ultrathin frozen sections were incubated with goat antiserum against rat osteocalcin at high dilutions (2×10−4 to 2×10−6). In calvaria and femur of untreated rats, endogenous osteocalcin was observed in osteoblasts (cytoplasm and nucleus) and in the collagenous matrix. Osteocalcin appeared progressively in osteoblasts and bone matrix in the mineralization front, then increased in the regions of extended calcification. Osteocalcin was also detected in osteocytes but was not as abundant as in osteoblasts. In bone samples of warfarin-treated rats, endogenous osteocalcin was only detected in bone matrix but not in osteoblasts. Furthermore, osteocalcin was only observed if antiserum was not very dilute (2×10−2). In cartilage (hypertrophied and degenerative zones), osteocalcin was not observed in matrix and chondrocytes. However, it was found in the vicinity of matrix vesicles at the initial loci of calcification. Osteocalcin was never detected in the cartilage of warfarin-treated rats. Our results provide ultrastructural immunocytological evidence for the localization of endogenous osteocalcin in osteoblasts, the presence of osteocalcin in bone matrix and a direct gradient between the presence of osteocalcin and the calcification process. Osteocalcin is absent from cartilage, except possibly close to calcifying matrix vesicles. Warfarin inhibits the formation of osteocalcin. Springer-Verlag, 1990 Osteocalcin nationallicence Osteoblast nationallicence Osteocyte nationallicence Ultrathin frozen sections nationallicence Warfarin treatment nationallicence Boivin Georges Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut Morel Gérard Faculté Lyon-Sud, CNRS URA 559, Oullins, France aut Lian Jane Laboratory for the Study of Skeletal Disorders, Harvard Medical School, Boston, USA aut Anthoine-Terrier Chantal Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut Dubois Paul Faculté Lyon-Sud, CNRS URA 559, Oullins, France aut Meunier Pierre Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut Virchows Archiv A Springer-Verlag 417/6(1990-11-01), 505-512 0174-7398 417:6<505 1990 417 428 https://doi.org/10.1007/BF01625731 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01625731 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boivin Georges Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut NATIONALLICENCE 700 1- Morel Gérard Faculté Lyon-Sud, CNRS URA 559, Oullins, France aut NATIONALLICENCE 700 1- Lian Jane Laboratory for the Study of Skeletal Disorders, Harvard Medical School, Boston, USA aut NATIONALLICENCE 700 1- Anthoine-Terrier Chantal Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut NATIONALLICENCE 700 1- Dubois Paul Faculté Lyon-Sud, CNRS URA 559, Oullins, France aut NATIONALLICENCE 700 1- Meunier Pierre Faculté A. Carrel, INSERM Unité 234, rue G. Paradin, F-69372, Lyon Cedex 08, France aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/6(1990-11-01), 505-512 0174-7398 417:6<505 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer