caa a22 4500 465753868 CHVBK 20180323111841.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01600133 doi (NATIONALLICENCE)springer-10.1007/BF01600133 Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland [Elektronische Daten] [F. O'Malley, D. Grignon, D. Shum] Summary: There are a number of benign, small-acinar lesions in the prostate gland that may be difficult to differentiate from small-acinar adenocarcinoma. An important diagnostic criterion in this differentiation is the loss of the basal layer in small-acinar adenocarcinoma and its preservation in benign conditions. A monoclonal antibody to high-molecular-weight cytokeratins (34βE12) has been shown to stain these basal cells preferentially. To assess the usefulness of this antibody in distinguishing benign from malignant small-acinar lesions, we examined 21 cases of small-acinar adenocarcinoma and 47 examples of benign lesions, which included atypical adenomatous hyperplasia, atrophy, post-sclerotic hyperplasia, basal cell hyperplasia, and fibroepithelial nodule. Positive staining with 34βEl2 was seen in 13/13 cases of atypical adenomatous hyperplasia, although in some cases the staining was weak and focal. Positivity with 34βE12 was also demonstrated in all other benign lesions studied. All 21 cases of small-acinar adenocarcinoma showed no reactivity with 34βEl2. The results suggest that 34βE12 is of value in distinguishing between well-differentiated, small-acinar prostatic adenocarcinoma and its mimics. However, care is needed in interpretation of staining in formalin-fixed material due to the variable reactivity, particularly in cases of atypical adenomatous hyperplasia. Springer-Verlag, 1990 Prostatic basal cells nationallicence Prostatic adenocarcinoma nationallicence High-molecular-weight cytokeratin nationallicence O'Malley F. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut Grignon D. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut Shum D. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut Virchows Archiv A Springer-Verlag 417/3(1990-05-01), 191-196 0174-7398 417:3<191 1990 417 428 https://doi.org/10.1007/BF01600133 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600133 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- O'Malley F. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut NATIONALLICENCE 700 1- Grignon D. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut NATIONALLICENCE 700 1- Shum D. Department of Pathology, Victoria Hospital, 375 South Street, N6A 4G5, London, Ontario, Canada aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/3(1990-05-01), 191-196 0174-7398 417:3<191 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer