caa a22 4500 465753981 CHVBK 20180323111842.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01605790 doi (NATIONALLICENCE)springer-10.1007/BF01605790 Immunoreactive luteinizing hormone in functioning corticotroph adenomas of the pituitary [Elektronische Daten] Immunohistochemical and tissue culture studies of two cases [Toshiaki Sano, Kalman Kovacs, Sylvia Asa, Harley Smyth] Summary: Two pituitary adenomas removed from a 37-year-old woman and a 26-year-old woman with typical Cushing's disease were studied by light and electron microscopy, immunohistochemistry and radioimmunoassay of tissue culture media. Both patients had high plasma levels of cortisol and normal levels of luteinizing hormone (LH). Both tumours were monomorphous, composed of densely granulated corticotrophs; the tumour cells contained periodic acid-Schiff positivity, were arranged in a sinusoidal pattern and, ultrastructurally, contained well-developed cytoplasmic organelles. By immunohistochemistry the majority of tumour cells contained immunoreactive adrenocorticotropin (ACTH); approximately 10% of the tumour cell population contained LH immunoreactivity. The LH-positive cells tended to form clusters scattered widely throughout the tumour tissues. LH immunoreactivity was demonstrated in some ACTH-immunoreactive cells on serial sections. Large amounts of immunoreactive ACTH and smaller quantities of LH, follicle stimulating hormone and αsubunit were released into the culture media and release of the glycoprotein hormones responded in parallel to corticotropin releasing hormone stimulation or inhibition by cortisol. These findings indicate that LH can be simultaneously produced and released by ACTH-producing tumour cells of otherwise typical functioning corticotroph adenomas. The capacity for LH production may be acquired during neoplastic proliferation. This is the first detailed report of concurrent production of LH by pituitary corticotroph adenomas. Springer-Verlag, 1990 Corticotroph adenoma nationallicence Adrenocorticotropin nationallicence Luteinizing hormone nationallicence Immunohistochemistry nationallicence Tissue culture nationallicence Sano Toshiaki Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut Kovacs Kalman Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut Asa Sylvia Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut Smyth Harley Department of Neurological Surgery, Wellesley Hospital, University of Toronto, Toronto, Canada aut Virchows Archiv A Springer-Verlag 417/4(1990-07-01), 361-367 0174-7398 417:4<361 1990 417 428 https://doi.org/10.1007/BF01605790 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605790 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sano Toshiaki Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut NATIONALLICENCE 700 1- Kovacs Kalman Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut NATIONALLICENCE 700 1- Asa Sylvia Department of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Canada aut NATIONALLICENCE 700 1- Smyth Harley Department of Neurological Surgery, Wellesley Hospital, University of Toronto, Toronto, Canada aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/4(1990-07-01), 361-367 0174-7398 417:4<361 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer