caa a22 4500 465754007 CHVBK 20180323111842.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01605778 doi (NATIONALLICENCE)springer-10.1007/BF01605778 Duan Hui-Jun Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi Matsumoto, 390, Nagano, Japan aut Sequential ultrastructural podocytic lesions and development of proteinuria in serum sickness nephritis in the rat [Elektronische Daten] [Hui-Jun Duan] Summary: Serum sickness nephritis was induced in Fisher rats by immunization with egg albumin (EA) and correlations between immune complex deposition, alterations of podocytes and development of proteinuria were analysed. Immunoelectron microscopy showed that EA, rat IgG and C3 were confined to the electron-dense deposits (Ds). From 3 weeks, when significant proteinuria had developed, the subepithelial region was filled with large numbers of Ds on the peripheral capillary wall as well as in the paramesangium. The loss of slit diaphragms and detachment of foot processes overlying Ds were observed and the escape of Ds into urinary space was frequently detected. Morphometric evaluation showed that the volume of subepithelial Ds and the number of the sites of podocytic detachment correlate significantly with the amount of proteinuria. In addition, the native ferritin injected via the abdominal aorta was seen in large amounts in the urinary space near the areas devoid of epithelial covering. The development of podocytic detachment was clearly coincident with the appearance of proteins with a larger molecular weight in urine. From these results, it is suggested that the loss of slit diaphragms and the detachment of podocytes resulting from the progressive accumulation of Ds will allow the leakage of proteins of larger molecular weight across the capillary wall. These podocytic lesions may be one of the main aetiologies for the development of heavy proteinuria in this model. Springer-Verlag, 1990 Serum sickness nephritis nationallicence Proteinuria nationallicence Immune complex nationallicence Podocyte detachment nationallicence Slit diaphragm nationallicence Virchows Archiv A Springer-Verlag 417/4(1990-07-01), 279-290 0174-7398 417:4<279 1990 417 428 https://doi.org/10.1007/BF01605778 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605778 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Duan Hui-Jun Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi Matsumoto, 390, Nagano, Japan aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/4(1990-07-01), 279-290 0174-7398 417:4<279 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer