caa a22 4500 465754058 CHVBK 20180323111842.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01606035 doi (NATIONALLICENCE)springer-10.1007/BF01606035 Human papillomavirus type 11DNA in papillary squamous cell lung carcinoma [Elektronische Daten] [F. Bejui-Thivolet, Y. Chardonnet, L. Patricot] Summary: We report a case of papillary squamous cell carcinoma of the lung developing in relation to a condylomatous papilloma and related to human papillomavirus (HPV) infection. The viral origin of the bronchial papillomatous lesion is strongly suggested by cytological and histological features with marked condylomatous changes. No viral capsid antigen was detected by immunohistochemistry. Transmission electron microscopy failed to reveal intranuclear viral-like particles in the papillary part of the carcinoma, but typical ultrastructural koilocytotic cells with irregular nucleus and coarse chromatin were observed. HPV DNA type 11 was detected by in situ hybridization using biotinylated probes on paraffin-embedded specimens, under stringent conditions (T m }-12‡, 50% formamide). Papillary squamous cell carcinoma may result from the malignant conversion of benign squamous papilloma of the bronchus. HPV type 11 may be associated with malignant conversion of benign papilloma of the pulmonary tract, as in the upper respiratory tract. In situ hybridization with biotinylated probes is a relatively simple and appropriate method for retrospective analysis of HPV DNA sequences in surgical specimens. Springer-Verlag, 1990 Human papillomavirus nationallicence Primary lung carcinoma nationallicence In situ hybridization nationallicence Biotinylated probes nationallicence Bejui-Thivolet F. Laboratoire d'Anatomie et de Cytologie Pathologiques HÔpital de la Croix-Rousse, F-69317, Lyon Cedex 04, France aut Chardonnet Y. INSERM U. 209 affiliée, CNRS, Pavillon R HÔpital Edouard Herriot, F-69437, Lyon Cedex 03, France aut Patricot L. Laboratoire d'Anatomie et de Cytologie Pathologiques HÔpital de la Croix-Rousse, F-69317, Lyon Cedex 04, France aut Virchows Archiv A Springer-Verlag 417/5(1990-09-01), 457-461 0174-7398 417:5<457 1990 417 428 https://doi.org/10.1007/BF01606035 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01606035 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bejui-Thivolet F. Laboratoire d'Anatomie et de Cytologie Pathologiques HÔpital de la Croix-Rousse, F-69317, Lyon Cedex 04, France aut NATIONALLICENCE 700 1- Chardonnet Y. INSERM U. 209 affiliée, CNRS, Pavillon R HÔpital Edouard Herriot, F-69437, Lyon Cedex 03, France aut NATIONALLICENCE 700 1- Patricot L. Laboratoire d'Anatomie et de Cytologie Pathologiques HÔpital de la Croix-Rousse, F-69317, Lyon Cedex 04, France aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/5(1990-09-01), 457-461 0174-7398 417:5<457 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer