caa a22 4500 465754236 CHVBK 20180323111842.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02190525 doi (NATIONALLICENCE)springer-10.1007/BF02190525 Immunocytochemical differential diagnosis of adrenocortical neoplasms using the monoclonal antibody D11 [Elektronische Daten] [Sören Schröder, Axel Mendorf, Eike Achilles, Manfred Dietel, Barbara Padberg, Ulrike Beisiegel, Henning Dralle, Max Bressel, Günter Klöppel] Summary: The monoclonal antibody D11 is a valuable aid in the accurate typing of adrenal tumours as, in formalin-fixed, paraffin-embedding material, strong nuclear D11 positivity was observed only in adrenocortical cells in 190 neoplasms (including 100 adrenal tumours). This pattern was demonstrated for all zona glomerulosa cells in 27 normal adrenals and for the neoplastic cells of 15 adrenocortical adenomas derived from that zone, as judged from clinically evident hyperaldosteronism. Normal cells of zona fasciculata and reticularis also showed strong diffuse D11 immunostaining and the same nuclear plus cytoplasmic D11 reactivity was evident in 15 benign and malignant adrenocortical neoplasms derived from these zones, documented by hypercortisolism. Cytoplasmic and/or nuclear D11 staining made topohistogenetic typing possible in 15 non-functioning cortical tumours. D11 immunostaining gave negative results in 50 specimens containing normal, hyperplastic and neoplastic adrenomedullary cells. In addition, absence of D11 reactivity was recorded in 4 adrenal metastases of extra-adrenal carcinomas, 5 paragangliomas, 25 primary renal carcinomas and 59 of 60 primary thyroid carcinomas. D11 immunocytochemistry allows the accurate typing of benign and malignant adrenocortical neoplasms, irrespective of histology and function. With this method, primary adrenocortical tumours can be separated from carcinomas metastatic to the adrenal gland, including secondary tumours of similar phenotype (such as renal carcinomas). By exclusion, D11 negativity provides evidence of the medullary origin of primary adrenal tumours even in the absence of clinical, structural, histochemical and conventional immunohistochemical indicators of phaeochromocytoma. Springer-Verlag, 1990 Adrenal gland nationallicence Adrenocortical tumours nationallicence Immunocytochemistry nationallicence Schröder Sören Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut Mendorf Axel Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut Achilles Eike Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut Dietel Manfred Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut Padberg Barbara Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut Beisiegel Ulrike Department of Internal Medicine, University Hospital Hamburg-Eppendorf, Hamburg, Germany aut Dralle Henning Department of Surgery, Hannover School of Medicine, Hannover, Germany aut Bressel Max Department of Urology, General Hospital Hamburg-Harburg, Hamburg, Germany aut Klöppel Günter Institute of Pathology, Free University of Brussels, Brussels, Belgium aut Virchows Archiv A Springer-Verlag 417/2(1990-06-01), 89-96 0174-7398 417:2<89 1990 417 428 https://doi.org/10.1007/BF02190525 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02190525 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schröder Sören Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut NATIONALLICENCE 700 1- Mendorf Axel Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut NATIONALLICENCE 700 1- Achilles Eike Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut NATIONALLICENCE 700 1- Dietel Manfred Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut NATIONALLICENCE 700 1- Padberg Barbara Institut für Pathologie, Univer-sität Hamburg (UKE), Martinistrasse 52, D-2000, Hamburg 20, Germany aut NATIONALLICENCE 700 1- Beisiegel Ulrike Department of Internal Medicine, University Hospital Hamburg-Eppendorf, Hamburg, Germany aut NATIONALLICENCE 700 1- Dralle Henning Department of Surgery, Hannover School of Medicine, Hannover, Germany aut NATIONALLICENCE 700 1- Bressel Max Department of Urology, General Hospital Hamburg-Harburg, Hamburg, Germany aut NATIONALLICENCE 700 1- Klöppel Günter Institute of Pathology, Free University of Brussels, Brussels, Belgium aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/2(1990-06-01), 89-96 0174-7398 417:2<89 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer