caa a22 4500 465754260 CHVBK 20180323111843.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02190532 doi (NATIONALLICENCE)springer-10.1007/BF02190532 DNA ploidy and cell-cycle analysis in pancreatic and ampullary carcinoma: flow cytometric study of formalin-fixed paraffin-embedded tissue [Elektronische Daten] [H. Baisch, G. Klöppel, Brigitta Reinke] Summary: The cellular DNA content of formalin-fixed, paraffin-embedded specimens from 47 ductal adenocarcinomas of the pancreas and 5 adenocarcinomas of the ampulla of Vater was analysed using flow cytometry. Ploidy and the fraction of cells in the S and G2M phases were determined and correlated with tumour stage and grade as well as patients' survival. Cell populations with aneuploid DNA content were observed in 15% of the tumours. The S + G2M fractions ranged between 1% and 10%. Compared to non-neoplastic tissue of the pancreas the S + G2M fraction was significantly higher in the carcinomas. Cox regression analysis revealed the S + G2M fraction as an independent prognostic factor (p< 0.05). Ploidy was of no prognostic value for survival, but correlated weakly with tumour stage and tumour grade. All patients without lymph node metastases at time of surgery had diploid tumours. Aneuploidy was restricted to tumours in advanced stages and tended to be more frequent in high-grade tumours. Springer-Verlag, 1990 Pancreatic adenocarcinoma nationallicence Prognosis nationallicence Aneuploidy nationallicence DNA index nationallicence Baisch H. Institute of Biophysics and Radiobiology, University of Hamburg, Hamburg, Germany aut Klöppel G. Department of Pathology, Free University of Brussels, Brussels, Belgium aut Reinke Brigitta Institute of Biophysics and Radiobiology, University of Hamburg, Hamburg, Germany aut Virchows Archiv A Springer-Verlag 417/2(1990-06-01), 145-150 0174-7398 417:2<145 1990 417 428 https://doi.org/10.1007/BF02190532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02190532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baisch H. Institute of Biophysics and Radiobiology, University of Hamburg, Hamburg, Germany aut NATIONALLICENCE 700 1- Klöppel G. Department of Pathology, Free University of Brussels, Brussels, Belgium aut NATIONALLICENCE 700 1- Reinke Brigitta Institute of Biophysics and Radiobiology, University of Hamburg, Hamburg, Germany aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/2(1990-06-01), 145-150 0174-7398 417:2<145 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer