caa a22 4500 465757316 CHVBK 20180323111850.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321115 doi (NATIONALLICENCE)springer-10.1007/BF00321115 Distant upstream regulatory sequences control the level of expression of the am (GDH) locus of Neurospora crassa [Elektronische Daten] [G. Frederick, J. Kinsey] Summary: We have constructed deletions in the 5′ non-coding sequences of the cloned Neurospora crassa am gene. Vectors with a truncated fragment of the am gene were used in transformation experiments to introduce the deletions into the chromosome by homologous recombination. Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression. The closer of these two elements (URSamα) is at approximately 1.4 kb upstream of the transcriptional start site. The second elements (URSamβ) is located between 2.1 and 3.2 kb upstream of the transcription start site. Deletion of either of these two elements reduces am expression to about 50% of the wild-type level. Deletion of both elements reduce am expression to from 5-16% of the wild-type level. Deletion of 1.1 kb of sequence just downstream of URSamα, which brings this element to within 300 bp of the transcription start site, had no effect on am expression. Likewise, deletion of 3.5 kb of sequence upstream of URSamβ had no effect on expression. None of these deletions had any effect on the expression of usg-1, a gene of unknown function that is transcribed in the same direction as the am gene, and which terminates about 3.5 kb upstream of the URSamβ element. Springer-Verlag, 1990 Transcription nationallicence Gene expression nationallicence In vitro mutagenesis nationallicence Gene replacement nationallicence Frederick G. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical School, 66103, Kansas City, KS, USA aut Kinsey J. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical School, 66103, Kansas City, KS, USA aut Current Genetics Springer-Verlag 18/1(1990-07-01), 53-58 0172-8083 18:1<53 1990 18 294 https://doi.org/10.1007/BF00321115 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321115 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frederick G. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical School, 66103, Kansas City, KS, USA aut NATIONALLICENCE 700 1- Kinsey J. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical School, 66103, Kansas City, KS, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 53-58 0172-8083 18:1<53 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer