caa a22 4500 465757324 CHVBK 20180323111850.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321112 doi (NATIONALLICENCE)springer-10.1007/BF00321112 Analysis of interchromosomal mitotic recombination [Elektronische Daten] [C. McGill, B. Shafer, D. Higgins, J. Strathern] Summary: A novel synthetic locus is described that provides a simple assay system for characterizing mitotic recombinants. The locus consists of the TRP1 and HIS3 genes inserted into chromosome III of S. cerevisiae between the CRY1 and MAT loci. Defined trp1 and his3 alleles have been generated that allow the selection of interchromosomal recombinants in this interval. Trp+ or His+ recombinants can be divided into several classes based on coupling of the other alleles in the interval. The tight linkage of the CRY1 and MAT loci, combined with the drug resistance and cell type phenotypes that they respectively control, facilitates the classification of the recombinants without resorting to tetrad dissection. We present the distribution of spontaneous recombinants among the classes defined by this analysis. The data suggest that the recombination intermediate can have regions of symmetric strand exchange and that co-conversion tracts can extend over 1-3 kb. Continuous conversion tracts are favored over discontinuous tracts. The distribution among the classes defined by this analysis is altered in recombinants induced by UV irradiation. Springer-Verlag, 1990 Recombination nationallicence DNA repair nationallicence UV irradiation nationallicence Yeast nationallicence McGill C. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut Shafer B. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut Higgins D. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut Strathern J. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut Current Genetics Springer-Verlag 18/1(1990-07-01), 29-39 0172-8083 18:1<29 1990 18 294 https://doi.org/10.1007/BF00321112 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321112 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McGill C. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 700 1- Shafer B. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 700 1- Higgins D. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 700 1- Strathern J. Basic Research Program, Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, P.O. Box B, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 29-39 0172-8083 18:1<29 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer