caa a22 4500 465757340 CHVBK 20180323111850.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321108 doi (NATIONALLICENCE)springer-10.1007/BF00321108 Expression of the avian gag-myc oncogene in Saccharomyces cerevisiae [Elektronische Daten] [P. Durrens, A. Fournier, L. Desfarges, M. Aigle] Summary: The gag-myc oncogenic sequence of the avian retrovirus MC29 was first inserted in a multicopy expression vector allowing its expression in Saccharomyces cerevisiae. The oncogene transcripts were detected in yeast by Northern blot hybridization and gag-myc proteins were revealed by immunoprecipitation. On solid medium, the average size of gag-myc transformant colonies was smaller than control. In liquid cultures, the gag-myc strains had a doubling time of 4.7 h compared with 3.1 h in the controls. In one of the recipient strains, and after an initial transient period of 5 days, the gag-myc transformants became physiologically indistinguishable from control. In another recipient strain, the slow-growth phenotype is permanent. Plasmid instability is increased in gag-myc transformants. When a single copy of the gag-myc gene was inserted in a yeast chromosome, no phenotype was observed, showing that slow growth is the consequence of plasmid loss. Springer-Verlag, 1990 Heterologous expression nationallicence myc oncogene nationallicence Yeast transformation nationallicence Durrens P. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut Fournier A. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut Desfarges L. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut Aigle M. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut Current Genetics Springer-Verlag 18/1(1990-07-01), 7-12 0172-8083 18:1<7 1990 18 294 https://doi.org/10.1007/BF00321108 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321108 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Durrens P. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut NATIONALLICENCE 700 1- Fournier A. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut NATIONALLICENCE 700 1- Desfarges L. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut NATIONALLICENCE 700 1- Aigle M. Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA 542, Allée des Facultés, F-33405, Talence-Cedex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 7-12 0172-8083 18:1<7 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer