caa a22 4500 465757367 CHVBK 20180323111850.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321122 doi (NATIONALLICENCE)springer-10.1007/BF00321122 Allelism of SNQ1 and ATR1 , genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide an aminotriazole [Elektronische Daten] [P. Gömpel-Klein, M. Brendel] Summary: SNQ1 gene function is required for the expression of resistance to 4NQO in wild-type yeast. The sequence of a 3.7 kb yeast DNA containing the gene SNQ1 was determined. The SNQ1 gene consists of an open reading frame of 1641 bp and encodes, according to the hydrophobicity analysis of the putative protein, a transmembrane protein of 547 amino acids. Homology searches in yeast genome databanks revealed a 100% sequence homology with gene ATR1 which controls resistance to aminotriazole in S. cerevisiae. Pre-treatment of wild-type yeast, but not of snq1-0::LEU2 disruption mutants, with sublethal doses of aminotriazole induced hyper-resistance to 4-nitroquinoline-N-oxide. Partial deletion of the nucleotide sequence coding for a putative ATP-binding site has no, or little, influence on resistance to 4NQO whereas total deletion of the region coding for this ATP-binding domain leads to 4NQO-sensitive nullmutants. Springer-Verlag, 1990 Mutagen resistance nationallicence DNA sequencing nationallicence Transmembrane protein nationallicence Gene disruption nationallicence 4NQO : 4-nitroquinoline-N-oxide nationallicence aminotriazole : 3-amino-1,2,4-triazole nationallicence HYR : hyper-resistance nationallicence Gömpel-Klein P. Institut für Mikrobiologie der J. W. Goethe-University, Theodor-Stern-Kai 7, Haus 75, D-6000, Frankfurt/Main, Federal Republic of Germany aut Brendel M. Institut für Mikrobiologie der J. W. Goethe-University, Theodor-Stern-Kai 7, Haus 75, D-6000, Frankfurt/Main, Federal Republic of Germany aut Current Genetics Springer-Verlag 18/1(1990-07-01), 93-96 0172-8083 18:1<93 1990 18 294 https://doi.org/10.1007/BF00321122 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321122 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gömpel-Klein P. Institut für Mikrobiologie der J. W. Goethe-University, Theodor-Stern-Kai 7, Haus 75, D-6000, Frankfurt/Main, Federal Republic of Germany aut NATIONALLICENCE 700 1- Brendel M. Institut für Mikrobiologie der J. W. Goethe-University, Theodor-Stern-Kai 7, Haus 75, D-6000, Frankfurt/Main, Federal Republic of Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 93-96 0172-8083 18:1<93 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer