caa a22 4500 465757375 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321110 doi (NATIONALLICENCE)springer-10.1007/BF00321110 Blockage to exonuclease III digestion in the chromatin of Saccharomyces cerevisiae maps to the in vitro — determined binding site of a trans-acting regulatory factor [Elektronische Daten] [W. Feaver, R. Pearlman] Summary: A series of transposable element-induced mutations at the HIS4 locus in Saccharomyces cerevisiae have been attributed to the transposition of a Ty element into the 5′ regulatory region of this gene. Various Ty-containing His+ revertants have been isolated and the HIS4/Ty junction region sequenced. The only difference found in this region between a His- and a weak His+ strain was a single point mutation, an A→G transition. The position of Ty remained unaltered. Examination of lacZ fusion plasmids further implicated this A→G transition as being reponsible for the altered phenotype, the bp transition representing an allele of a cis-acting regulatory element. Subsequent gel retardation and methylation interference experiments revealed that this A→G mutation enabled the binding of a trans-acting factor (TyBf) in vitro. In this paper we show that the TyBf binding site is in a region of chromatin hypersensitive to digestion by DNase I. The binding site is protected in vivo from digestion with exonuclease III, suggesting the presence of a bound protein in His+ ("on”) but not His- ("off”) Ty-containing strains. We propose that a trans-acting factor binding in vivo, presumably TyBf, is responsible for the activation of HIS4 expression in these insertion mutants. Springer-Verlag, 1990 Yeast nationallicence Ty2 nationallicence Protein/DNA binding nationallicence Transcription nationallicence Feaver W. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Toronto, Ontario, Canada aut Pearlman R. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Toronto, Ontario, Canada aut Current Genetics Springer-Verlag 18/1(1990-07-01), 17-22 0172-8083 18:1<17 1990 18 294 https://doi.org/10.1007/BF00321110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feaver W. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Pearlman R. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 17-22 0172-8083 18:1<17 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer