caa a22 4500 465757383 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321119 doi (NATIONALLICENCE)springer-10.1007/BF00321119 Killer toxin production in Pichia acaciae is associated with linear DNA plasmids [Elektronische Daten] [P. Worsham, P. Bolen] Summary: We have identified a strain of the yeast Pichia acaciae which produces a "killer” toxin active against the yeast Debaryomyces tamarii. The killer phenotype was associated with the presence of two DNA plasmids, pPacl-1 (13.6 kilobase pairs) and pPacl-2 (7.3 kilobase pairs). P. acaciae strains, cured of these plasmids by irradiation with ultraviolet light, lacked killer activity and were sensitive to toxin produced by the parental strain. A partially cured strain, GS-1215, missing only the smaller plasmid, pPacl-2, also exhibited loss of both toxin activity and immunity. Exonuclease studies revealed that both plasmids were linear double-stranded DNA molecules with 5′ protected ends. The P. acaciae system differs from that of the well-studied Kluyveromyces lactis "killer” system both in the range of susceptible strains and in the sizes of the plasmids involved. Our studies contradict previous reports that Pichia killer systems are invariably chromosomal. Springer-Verlag, 1990 Yeast Pichia acaciae nationallicence Killer toxin nationallicence Extrachromosomal DNA nationallicence Worsham P. Microbial Properties Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, USA aut Bolen P. Microbial Properties Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, USA aut Current Genetics Springer-Verlag 18/1(1990-07-01), 77-80 0172-8083 18:1<77 1990 18 294 https://doi.org/10.1007/BF00321119 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321119 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Worsham P. Microbial Properties Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, USA aut NATIONALLICENCE 700 1- Bolen P. Microbial Properties Research, Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 77-80 0172-8083 18:1<77 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer