caa a22 4500 465757405 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321118 doi (NATIONALLICENCE)springer-10.1007/BF00321118 The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG -negative mutant strain [Elektronische Daten] [F. Gruber, J. Visser, C. Kubicek, L. de Graaff] Summary: Six uridine auxotroph mutants of Trichoderma reesei QM 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as OMP-decarboxylase negative (pyr -) by a radiometric enzyme assay. Transformation to uridine prototrophy was achieved with the pyr4 gene of Neurospora crassa (up to 1500 transformants/μg) and with pyrA of Aspergillus niger (700-800 transformants/μg). In many transformants the PYR+ function seems to be present as extrachromosomal DNA. There is evidence for a correlation between the stability of transformants and integration of the vector in the genome whereas unstable transformants are obtained when autonomous replication of the plasmid occurs. Springer-Verlag, 1990 Trichoderma reesei nationallicence Transformation nationallicence OMP-decarboxylase nationallicence Vector integration nationallicence Autonomous replication nationallicence Gruber F. Abt Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut Visser J. Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Kubicek C. Abt Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut de Graaff L. Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Current Genetics Springer-Verlag 18/1(1990-07-01), 71-76 0172-8083 18:1<71 1990 18 294 https://doi.org/10.1007/BF00321118 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321118 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gruber F. Abt Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut NATIONALLICENCE 700 1- Visser J. Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kubicek C. Abt Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut NATIONALLICENCE 700 1- de Graaff L. Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 71-76 0172-8083 18:1<71 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer