caa a22 4500 465757413 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00321117 doi (NATIONALLICENCE)springer-10.1007/BF00321117 Sealy-Lewis H. Department of Applied Biology, University of Hull, HU6 7RX, Hull, UK aut The identification of mutations in Aspergillus nidulans that lead to increased levels of ADHII [Elektronische Daten] [H. Sealy-Lewis] Summary: There are at least three alcohol dehydrogenases in Aspergillus nidulans. ADHII has been observed in polyacrylamide gels stained for ADH activity but, unlike ADHI and ADHIII, no physiological function has been attributed to it. This paper describes mutations that have been isolated from strains carrying a deletion in the structural gene for ADHI (alcA) and its adjacent positively-acting regulatory gene (alcR) that restore some ability to utilise ethanol as a carbon source. The mutations map at three loci, and all show elevated levels of the ADHII staining band. An assay for ADHII has been developed. The growth on ethanol has been shown to be dependent on the previously identified aldehyde dehydrogenase (structural gene, aldA). Two of the mutations, alcD and alcE, represent newly discovered mutations affecting ethanol utilisation while the third mutation is in amdA, a previously described trans-acting regulatory protein. Springer-Verlag, 1990 Aspergillus nidulans nationallicence ADHII nationallicence Current Genetics Springer-Verlag 18/1(1990-07-01), 65-70 0172-8083 18:1<65 1990 18 294 https://doi.org/10.1007/BF00321117 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321117 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sealy-Lewis H. Department of Applied Biology, University of Hull, HU6 7RX, Hull, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/1(1990-07-01), 65-70 0172-8083 18:1<65 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer