caa a22 4500 465757456 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327021 doi (NATIONALLICENCE)springer-10.1007/BF00327021 Use of the Tn 903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe [Elektronische Daten] [Christine Lang-Hinrichs, Claudia Dössereck, Isabelle Fath, Ulf Stahl] Summary: The bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro aminoglycoside phosphotransferase activity. On the other hand, the heterologous promoter of the Saccharomyces cerevisiae alcohol dehydrogenase I gene (adc1) is shown to be a weak promoter in Schizosaccharomyces pombe, though its activity is significantly enhanced in cells grown on glycerol as a carbon source. This system for selection and detection of promoter-active sequences may provide a useful basis for the analysis of promoter elements in fission yeast. Springer-Verlag, 1990 Schizosaccharomyces pombe nationallicence S. cerevisiae adc1 promoter nationallicence S. pombe adh1 promoter nationallicence Aminoglycoside phosphotransferase nationallicence Lang-Hinrichs Christine Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut Dössereck Claudia FG Mikrobiologie und Genetik, Technische Universität Berlin, Gustav-Meyer-Allee 25, W-1000, Berlin 65, Germany aut Fath Isabelle Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut Stahl Ulf Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut Current Genetics Springer-Verlag 18/6(1990-12-01), 511-516 0172-8083 18:6<511 1990 18 294 https://doi.org/10.1007/BF00327021 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327021 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lang-Hinrichs Christine Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut NATIONALLICENCE 700 1- Dössereck Claudia FG Mikrobiologie und Genetik, Technische Universität Berlin, Gustav-Meyer-Allee 25, W-1000, Berlin 65, Germany aut NATIONALLICENCE 700 1- Fath Isabelle Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut NATIONALLICENCE 700 1- Stahl Ulf Institut für Gärungsgewerbe und Biotechnologie, Seestrasse 13, W-1000, Berlin 65, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 511-516 0172-8083 18:6<511 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer