caa a22 4500 465757464 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327025 doi (NATIONALLICENCE)springer-10.1007/BF00327025 Regulation of the glaA gene of Aspergillus niger [Elektronische Daten] [Timothy Fowler, Randy Berka, Michael Ward] Summary: The glucoamylase gene of Aspergillus niger, glaA, is expressed at high levels in the presence of starch. We have determined the nucleotide sequence of 1966 bp of the 5′ flanking region of the glaA gene and have begun to identify sequences important for the control of glaA expression by deletion analysis. Constructs containing deletions extending into the glaA gene promotor were introduced into an A. niger host whose own glaA gene had been disrupted by a gene replacement event. Secreted levels of glucoamylase, expressed from each of the recombinant glaA genes, were measured by enzyme immunoassay. The effect of each deletion on the expression of glaA, when grown on differering carbon sources, was used to determine the limits of sequences upstream of glaA responsible for gene regulation. A region between -562 and -318 appears to direct high-level expression, whereas only 214 bp of 5′ flanking sequence is required to initiate the start to transcription. Springer-Verlag, 1990 Aspergillus nationallicence Glucoamylase nationallicence Upstream sequence nationallicence Gene regulation nationallicence Fowler Timothy Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut Berka Randy Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut Ward Michael Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut Current Genetics Springer-Verlag 18/6(1990-12-01), 537-545 0172-8083 18:6<537 1990 18 294 https://doi.org/10.1007/BF00327025 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fowler Timothy Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Berka Randy Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 700 1- Ward Michael Genencor International, 180 Kimball Way, 94080, South San Francisco, CA, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 537-545 0172-8083 18:6<537 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer