caa a22 4500 465757499 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327028 doi (NATIONALLICENCE)springer-10.1007/BF00327028 Red algal plasmids [Elektronische Daten] [Lynda Goff, Annette Coleman] Summary: Five of 21 red algal genera were found to contain circular dsDNA plasmids, typically of two or more sizes per species. Clones of the two plasmids (GL4.4 and GL3.5 kbp), characterizing all isolates of Gracilariopsis lemaneiformis, do not cross-hybridize with each other, with the nuclear, plastid or mitochondrial genomes of G. lemaneiformis, or with any DNA genomes of the other red algae examined. Clones of both plasmids hybridized with discrete bands on Northern blots of total RNA and poly(A)+ RNA. Sequencing of the G. lemaneiformis 3.5 kbp plasmid revealed two potential open reading frames which, when used to probe Northern blots, confirmed the presence of specific transcripts. These autonomously replicating plasmids are present in high copy number per cell and in constant proportion to each other. Their constancy suggests a function of significance to the species. Red algal plasmids may provide useful vectors for transforming economically important red algal species. Springer-Verlag, 1990 Biotechnology nationallicence DNA sequence nationallicence Plasmid nationallicence Rhodophyta nationallicence Goff Lynda Department of Biology, University of California, 95064, Santa Cruz, CA, USA aut Coleman Annette Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut Current Genetics Springer-Verlag 18/6(1990-12-01), 557-565 0172-8083 18:6<557 1990 18 294 https://doi.org/10.1007/BF00327028 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goff Lynda Department of Biology, University of California, 95064, Santa Cruz, CA, USA aut NATIONALLICENCE 700 1- Coleman Annette Division of Biology and Medicine, Brown University, 02912, Providence, RI, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 557-565 0172-8083 18:6<557 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer