caa a22 4500 465757502 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327022 doi (NATIONALLICENCE)springer-10.1007/BF00327022 Centromeric DNA of Kluyveromyces lactis [Elektronische Daten] [Joris Heus, Ben Zonneveld, H. Steensma, Johan Van den Berg] Summary: A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number or approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae. Springer-Verlag, 1990 Isolation nationallicence Centromeres nationallicence Kluyveromyces lactis nationallicence Heus Joris Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut Zonneveld Ben Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut Steensma H. Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut Van den Berg Johan Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut Current Genetics Springer-Verlag 18/6(1990-12-01), 517-522 0172-8083 18:6<517 1990 18 294 https://doi.org/10.1007/BF00327022 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heus Joris Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Zonneveld Ben Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Steensma H. Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Van den Berg Johan Department of Cell Biology and Genetics, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 517-522 0172-8083 18:6<517 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer