caa a22 4500 465757510 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327026 doi (NATIONALLICENCE)springer-10.1007/BF00327026 Phylogenetic analysis of the RNA polymerases of Trypanosoma brucei , with special reference to class-specific transcription [Elektronische Daten] [Waldemar Jess, Peter Palm, Raymond Evers, Josef Köck, Albert Cornelissen] Summary: We have sequenced the genes encoding te largest subunits of the three classes of DNA-dependent RNA polymerases of Trypanosoma brucei. The nucleotide and deduced amino acid sequences were compared and aligned with the corresponding sequences of other eukaryotes. Phylogenetic relationships were subsequently calculated with a distant matrix, a bootstrapped parsimony and a maximum-likelihood method. These independent calculations resulted in trees with very similar topologies. The analyses show that all the largest subunits of T. brucei are evolutionarily distant members within each of the three RNA polymerase classes. An early separation of the trypanosomal subunits from the eukaryotic lineage might from the fundamental basis for the unusual transcription process of this species. Finally, all dendrograms show a separate ramification for the largest subunit of RNA polymerase I, II and III. RNA polymerase II and/or III form a bifurcation with the archaebacterial lineage. RNA polymerase I, however, arises separately from the eubacterial β′ lineage. This suggests that the three eukaryotic RNA polymerase classes are not simply derived by two gene duplications of an ancestral gene with subsequent differentiation. Springer-Verlag, 1990 Trypanosomes nationallicence RNA polymerase nationallicence Transcription nationallicence Evolution nationallicence Phylogeny nationallicence Jess Waldemar Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut Palm Peter Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, W-8033, Martinsried, Germany aut Evers Raymond Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut Köck Josef Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut Cornelissen Albert Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut Current Genetics Springer-Verlag 18/6(1990-12-01), 547-551 0172-8083 18:6<547 1990 18 294 https://doi.org/10.1007/BF00327026 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327026 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jess Waldemar Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Palm Peter Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, W-8033, Martinsried, Germany aut NATIONALLICENCE 700 1- Evers Raymond Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Köck Josef Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Cornelissen Albert Molecular Parasitology Unit, Max-Planck-Institut für Biologie, Spemannstraße 34, W-7400, Tübingen, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 547-551 0172-8083 18:6<547 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer