caa a22 4500 465757537 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327018 doi (NATIONALLICENCE)springer-10.1007/BF00327018 Ty virus-like particles in the Saccharomyces cerevisiae strain NCYC74 [Elektronische Daten] [Linda Capsey, Donald Williamson, Geoffrey Banks] Summary: Electron microscopic analysis of thin sections of Saccharomyces cerevisiae NCYC74 has revealed the presence of many clumped cytoplasmic particles that morphologically resemble Ty element virus-like particles (VLPs). Accumulation of Ty VLPs has only previously been observed in S. cerevisiae strains that over-express a cloned Ty element. The particles in NCYC74 co-purify with Ty RNA, Ty-specific antigens and a reverse transcriptase activity. Furthermore, they appear to be recognised by antibodies to Ty VLPs during indirect immunofluorescence experiments. These observations provide compelling evidence that the cytoplasmic particle in NCYC74 are indeed Ty VLPs. Springer-Verlag, 1990 Yeast nationallicence Ty elements nationallicence Virus like particles nationallicence Capsey Linda Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut Williamson Donald Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut Banks Geoffrey Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut Current Genetics Springer-Verlag 18/6(1990-12-01), 485-491 0172-8083 18:6<485 1990 18 294 https://doi.org/10.1007/BF00327018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Capsey Linda Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut NATIONALLICENCE 700 1- Williamson Donald Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut NATIONALLICENCE 700 1- Banks Geoffrey Genetics Division, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, England aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 485-491 0172-8083 18:6<485 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer