caa a22 4500 465757545 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00327019 doi (NATIONALLICENCE)springer-10.1007/BF00327019 Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2 , and construction of a xylose-utilizing Saccharomyces cerevisiae transformant [Elektronische Daten] [Peter Kötter, René Amore, Cornelis Hollenberg, Michael Ciriacy] Summary: A P. stipitis cDNA library in λgt11 was screened using antisera against P. stipitis xylose reductase and xylitol dehydrogenase, respectively. The resulting cDNA clones served as probes for screening a P. stipitis genomic library. The genomic XYL2 gene was isolated and the nucleotide sequence of the 1089 bp structural gene, and of adjacent non-coding regions, was determined. The XYL2 open-reading frame codes for a protein of 363 amino acids with a predicted molecular mass of 38.5 kDa. The XYL2 gene is actively expressed in S. cerevisiae transformants. S. cerevisiae cells transformed with a plasmid, pRD1, containing both the xylose reductase gene (XYL1) and the xylitol dehydrogenase gene (XYL2), were able to grow on xylose as a sole carbon source. In contrast to aerobic glucose metabolism, S. cerevisiae XYL1-XYL2 transformants utilize xylose almost entirely oxidatively. Springer-Verlag, 1990 Xylitol dehydrogenase gene nationallicence Pichia stipitis nationallicence Saccharomyces cerevisiae nationallicence Xylose utilization nationallicence Kötter Peter Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut Amore René Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut Hollenberg Cornelis Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut Ciriacy Michael Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut Current Genetics Springer-Verlag 18/6(1990-12-01), 493-500 0172-8083 18:6<493 1990 18 294 https://doi.org/10.1007/BF00327019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00327019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kötter Peter Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Amore René Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Hollenberg Cornelis Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Ciriacy Michael Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätstrasse 1, W-4000, Düsseldorf, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/6(1990-12-01), 493-500 0172-8083 18:6<493 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer