caa a22 4500 46575757X CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318389 doi (NATIONALLICENCE)springer-10.1007/BF00318389 Villemur R. Atlantic Research Laboratory, National Research Council of Canada, 1411 Oxford Street, B3H 3Z1, Halifax, Nova Scotia, Canada aut Circular plasmid DNAs from the red alga Gracilaria chilensis [Elektronische Daten] [R. Villemur] Summary: Total cellular DNA extracted from eight red algal species (from the genera Gracilaria, Gracilariopsis, Porphyra and Gymnogongrus) was centrifuged on Hoechst dye/CsCl gradients. In five species, plasmid-like DNAs banded with the A+T rich organellar DNAs in the CsCl gradients. Based on their electrophoretic migration in different agarose gels, the plasmid-like DNAs are circular. This is the first report of putative plasmid DNAs in the red algae outside the genus Gracilaria. Two similar Gracilaria chilensis plasmid-like DNAs of 3.8 and 3.4 kb (GC2 and GC3) were cloned in pUC19. The cloned GC2 DNA did not hybridize to either the organellar or nuclear genomes of G. chilensis, suggesting that GC2 is a true plasmid. GC2 did hybridize to the plasmid of one other red algal species, Gracilaria sordida. Springer-Verlag, 1990 Rhodophyta nationallicence Gracilaria nationallicence Eukaryotic plasmids nationallicence Current Genetics Springer-Verlag 18/3(1990-10-01), 251-257 0172-8083 18:3<251 1990 18 294 https://doi.org/10.1007/BF00318389 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318389 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Villemur R. Atlantic Research Laboratory, National Research Council of Canada, 1411 Oxford Street, B3H 3Z1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 251-257 0172-8083 18:3<251 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer