caa a22 4500 465757588 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318384 doi (NATIONALLICENCE)springer-10.1007/BF00318384 Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus [Elektronische Daten] [Toshihiko Ooi, Atsuhiko Shinmyo, Hirosuke Okada, Saburo Hara, Tokuji Ikenaka, Sawao Murao, Motoo Arai] Summary: Wa have cloned and characterized the cDNA coding for a major component of cellulase, endoglucanase (FI-CMCase), produced by Aspergillus aculeatus. The cDNA was isolated from a A. aculeatus cDNA library using synthetic oligonuceotide mixtures that correspond to the internal amino acid sequence of the mature FI-CMCase protein. Nucleotide sequence analysis of the cloned cDNA insert revealed a 711 bp open reading frame that encoded a protein of 237 amino acid residues. The primary structure of FI-CMCase deduced from the nucleotide sequence of cDNA agreed with that found by amino acid sequencing of peptide fragments obtained by digestion with several proteinases and cyanogen bromide cleavage. There may be a signal peptide sequence of 16 amino acid residues at the N-terminus. The molecular mass of the mature protein calculated from the cDNA is 24002 daltons, which compares favorably with molecular mass estimates of purified FI-CMCase obtained from SDS-PAGE (25000 Da). No distinct homology was found between the amino acid sequence of FI-CMCase and known cellulase sequences of other microorganisms. This study is the first example of cDNA cloning of an endoglucanase from the genus Aspergillus. Springer-Verlag, 1990 Cellulase nationallicence Filamentous fungi nationallicence Aspergillus aculeatus nationallicence cDNA cloning nationallicence Ooi Toshihiko Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut Shinmyo Atsuhiko Department of Fermentation Technology, Osaka University, Osaka, Japan aut Okada Hirosuke Department of Fermentation Technology, Osaka University, Osaka, Japan aut Hara Saburo Department of Chemistry, Osaka University, Osaka, Japan aut Ikenaka Tokuji Department of Chemistry, Osaka University, Osaka, Japan aut Murao Sawao Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut Arai Motoo Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut Current Genetics Springer-Verlag 18/3(1990-10-01), 217-222 0172-8083 18:3<217 1990 18 294 https://doi.org/10.1007/BF00318384 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318384 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ooi Toshihiko Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Shinmyo Atsuhiko Department of Fermentation Technology, Osaka University, Osaka, Japan aut NATIONALLICENCE 700 1- Okada Hirosuke Department of Fermentation Technology, Osaka University, Osaka, Japan aut NATIONALLICENCE 700 1- Hara Saburo Department of Chemistry, Osaka University, Osaka, Japan aut NATIONALLICENCE 700 1- Ikenaka Tokuji Department of Chemistry, Osaka University, Osaka, Japan aut NATIONALLICENCE 700 1- Murao Sawao Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Arai Motoo Department of Agricultural Chemistry, University of Osaka Prefecture, 591, Sakai-shi, Osaka, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 217-222 0172-8083 18:3<217 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer