caa a22 4500 46575760X CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318390 doi (NATIONALLICENCE)springer-10.1007/BF00318390 Detection of chloroplast DNA by using fluorescent monoclonal anti-bromodeoxyuridine antibody and analysis of its fate during zygote formation in Chlamydomonas reinhardtii [Elektronische Daten] [C. Munaut, D. Dombrowicz, R. Matagne] Summary: A monoclonal anti-bromodeoxyuridine antibody conjugated to fluorescein was used to detect the chloroplast nucleoids after specific incorporation of bromodeoxyuridine (BUdR) into the chloroplast DNA of Chlamydomonas reinhardtii. The incorporation of BUdR was enhanced by simultaneous treatment with fluorodeoxyuridine (FUdR). The method was applied to analyze the fate of chloroplast DNA in zygotes resulting from mating between BUdR-treated gametes (mt + or mt -) and untreated gametes of opposite mating-type. In crosses between wild-type strains, the nucleoids of mt + origin remained in the large majority of zygotes whereas those of mt - origin most often disappeared within the first hours following copulation. In crosses of the type mat-3 mt +xwild-type mt - (the mat-3 mutation permits a high transmission of chloroplast genes from the mt - parent), the nucleoids of mt - origin were generally not eliminated which indicates that the mat-3 mutation prevents the selective destruction of paternal chloroplast DNA in the zygote. Springer-Verlag, 1990 Chloroplast DNA nationallicence Monoclonal anti-bromodeoxyuridine antibody nationallicence Chlamydomonas nationallicence Munaut C. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut Dombrowicz D. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut Matagne R. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut Current Genetics Springer-Verlag 18/3(1990-10-01), 259-263 0172-8083 18:3<259 1990 18 294 https://doi.org/10.1007/BF00318390 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318390 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Munaut C. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut NATIONALLICENCE 700 1- Dombrowicz D. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut NATIONALLICENCE 700 1- Matagne R. Genetics of Microorganisms, Department of Botany, University of Liège, Sart Tilman, B 4000, Liege, Belgium aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 259-263 0172-8083 18:3<259 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer