caa a22 4500 465757634 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318393 doi (NATIONALLICENCE)springer-10.1007/BF00318393 Restriction site and genetic map of Cucurbita pepo chloroplast DNA [Elektronische Daten] [H. Lim, I. Gounaris, R. Hardison, C. Boyer] Summary: A detailed restriction map of squash chloroplast DNA (cpDNA) was constructed with five restriction endonuclease, SalI, PvuII, BglI, SacII, and PstI. The cleavage sites were mapped by sequential digestion of cpDNA using low-gelling temperature agarose. The restriction map shows that squash cpDNA is an approximately 153 kilobase (kb) circle with a large inverted repeat sequence of 23.3 kb, separated by a large (83.7 kb) and a small (22.7 kb) single copy region. Genes for a number of chloroplast polypeptides were localized on the map by hybridizing the cpDNA restriction fragments to heterologous gene-specific probes from tobacco, pea, tomato, maize, and spinach chloroplasts. The gene locations and organization of squash cpDNA are highly conserved and similar to chloroplast genomes of tomato, pepper, and Ginkgo. Springer-Verlag, 1990 Cucurbita pepo nationallicence Chloroplast DNA nationallicence Restriction map nationallicence Gene location nationallicence cpDNA : chloroplast DNA nationallicence kb : kilobases nationallicence IR : inverted repeat. Gene names follow the nomenclature recommendation of Hallick and Bottomley (1983) nationallicence Lim H. Department of Horticulture, The Pennsylvania State University, 16802, University Park, USA aut Gounaris I. USDA Regional Pasture Research Laboratory, The Pennsylvania State University, 16802, University Park, USA aut Hardison R. Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, USA aut Boyer C. Department of Horticulture, The Pennsylvania State University, 16802, University Park, USA aut Current Genetics Springer-Verlag 18/3(1990-10-01), 273-275 0172-8083 18:3<273 1990 18 294 https://doi.org/10.1007/BF00318393 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318393 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lim H. Department of Horticulture, The Pennsylvania State University, 16802, University Park, USA aut NATIONALLICENCE 700 1- Gounaris I. USDA Regional Pasture Research Laboratory, The Pennsylvania State University, 16802, University Park, USA aut NATIONALLICENCE 700 1- Hardison R. Department of Molecular and Cell Biology, The Pennsylvania State University, 16802, University Park, USA aut NATIONALLICENCE 700 1- Boyer C. Department of Horticulture, The Pennsylvania State University, 16802, University Park, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 273-275 0172-8083 18:3<273 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer