caa a22 4500 465757642 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318391 doi (NATIONALLICENCE)springer-10.1007/BF00318391 Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells [Elektronische Daten] [P. Skowronek, G. Krummeck, O. Haferkamp, G. Rödel] Summary: The cationic lipophilic dye Rhodamine 123 (Rh123) is selectively enriched in mitochondria in a membrane potential-dependent manner. Application of drugs which interfere with the electron flow of the respiratory chain lead to a severe reduction of mitochondrial dye uptake. In this communication we show that the same effect is observed after Rh123-staining of respiratory-deficient yeast mutants. Based on this observation we used flow cytometry to discriminate respiratory-compentent and respiratory-deficient yeast cells. Combined with a cell sorter we were able to selectively enrich respiring and non-respiring yeast cells, repectively, from a mixture of cells. Springer-Verlag, 1990 Flow cytometry nationallicence Rhodamine 123 nationallicence Respiratory chain nationallicence Yeast nationallicence Skowronek P. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut Krummeck G. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut Haferkamp O. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut Rödel G. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut Current Genetics Springer-Verlag 18/3(1990-10-01), 265-267 0172-8083 18:3<265 1990 18 294 https://doi.org/10.1007/BF00318391 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318391 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skowronek P. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Krummeck G. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Haferkamp O. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Rödel G. Institut für Pathologie der Universität Ulm, Oberer Eselsberg M23, D-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 265-267 0172-8083 18:3<265 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer