caa a22 4500 465757650 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318381 doi (NATIONALLICENCE)springer-10.1007/BF00318381 Detection of length heterogeneity in the ribosomal DNA of Pythium ultimum by PCR amplification of the intergenic region [Elektronische Daten] [J. Buchko, G. Klassen] Summary: About one-half of the ribosomal repeat unit of two isolates of Pythium ultimum was amplified by means of the polymerase chain reaction using one primer pair. The amplified region includes a small part of the large subunit ribosomal RNA gene, about half of the small subunit ribosomal RNA gene, and the entire intergenic region. The intergenic region of both isolates of P. ultimum has length heterogeneity due to the presence of subrepeat arrays (Klassen and Buchko 1990). PCR amplification of the heterogeneous target DNA resulted in sets of fragments which accurately reflect the heterogeneity in the target DNA, although there is a preferential amplification of the smaller targets. PCR product sizes ranged from 4.6 to 5.8 kb. Springer-Verlag, 1990 rDNA repeat nationallicence Pythium ultimum nationallicence Intergenic region heterogeneity nationallicence Polymerase chain reaction nationallicence Buchko J. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada aut Klassen G. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada aut Current Genetics Springer-Verlag 18/3(1990-10-01), 203-205 0172-8083 18:3<203 1990 18 294 https://doi.org/10.1007/BF00318381 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318381 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buchko J. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Klassen G. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 203-205 0172-8083 18:3<203 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer