caa a22 4500 465757669 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318385 doi (NATIONALLICENCE)springer-10.1007/BF00318385 Molecular organisation of an A mating type factor of the basidiomycete fungus Coprinus cinereus [Elektronische Daten] [E. Mutasa, A. Tymon, B. Göttgens, F. Mellon, P. Little, L. Casselton] Summary: The Aα3 and Aβ3 genes, which together constitute the A42 mating type factor of Coprinus cinereus, were isolated from a cosmid genomic library by walking 50 kb, a map distance of 0.5 units, from the closely linked metabolic gene pab-1. Cosmid clones having A gene function were identified by transformation into compatible A6 (α2β2) and A5 (α1β1) host cells where either α3 or β3 was expected to elicit the A factor — regulated development of unfused clamp cells. DNAs were digested with various enzymes before transformation in order to identify the smallest fragments containing an active α3 or β3 gene. Two non-overlapping fragments were identified as containing the α3 and β3 genes respectively. Southern hybridisation analyses showed that these two cloned genes had no detectable sequence homology, and that there was little or no hybridisation to the α and β gene alleles that constitute the A5 and A6 factors. α3 and β3 were shown to be less than 2.0 kb apart and embedded in a DNA sequence extending over 9.0 kb which was unique to our A42 strain and may contain a third A factor gene. Springer-Verlag, 1990 A factor cloning nationallicence Basidiomycetes nationallicence Coprinus nationallicence Mating type genes nationallicence Mutasa E. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Tymon A. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Göttgens B. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Mellon F. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Little P. Department of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AZ, London, UK aut Casselton L. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut Current Genetics Springer-Verlag 18/3(1990-10-01), 223-229 0172-8083 18:3<223 1990 18 294 https://doi.org/10.1007/BF00318385 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318385 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mutasa E. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Tymon A. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Göttgens B. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Mellon F. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Little P. Department of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AZ, London, UK aut NATIONALLICENCE 700 1- Casselton L. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 223-229 0172-8083 18:3<223 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer