caa a22 4500 465757677 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318387 doi (NATIONALLICENCE)springer-10.1007/BF00318387 Structure and expression of tomato mitochondrial genes coding for tRNACys (GCA), tRNAAsn (GUU) and tRNATyr (GUA): A native tRNACys gene is present in dicot plants but absent in monocot plants [Elektronische Daten] [Shinichi Izuchi, Tohru Terachi, Masahiro Sakamoto, Tetsuo Mikami, Mamoru Sugita] Summary: The nucleotide sequences of tRNAAsn (GUU) and tRNATyr (GUA) genes from tomato mitochondria and their flanking regions have been determined. The tomato mitochondrial tRNAAsn gene is located 2.1 kb downstream from the tRNACys gene reported previously (Izuchi and Sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. The tRNATyr gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a "native” mitochondrial tRNA gene and is 535 bp from the "chloroplast-like” tRNAAsn gene on the same strand. Northern hybridization analysis revealed that the three tRNA genes are transcribed in tomato mitochondria. Southern hybridization analysis of tomato, sugar beet, rice and wheat mitochondrial DNAs, with oligonucleotide probes for mitochondrial or chloroplast tRNA genes, demonstrated that the mitochondrial tRNACys gene found in tomato is present in dicot plants but not in monocots. On the other hand, a chloroplast-like tRNACys gene exists in monocot plants. Springer-Verlag, 1990 Mitochondria nationallicence tRNA genes nationallicence Tomato nationallicence Transcription nationallicence Izuchi Shinichi Department of Botany, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut Terachi Tohru Institute for National Land Utilization and Development, Kyoto Sangyo University, 603, Kyoto, Japan aut Sakamoto Masahiro Plant Biotechnology Department, Life Science Laboratory, Mitsui Toatsu Chemicals, Inc, 297, Mobara, Japan aut Mikami Tetsuo Laboratory of Industrial Crops, Faculty of Agriculture, Hokkaido University, 060, Sapporo, Japan aut Sugita Mamoru Center for Gene Research, Nagoya University, 464-01, Nagoya, Japan aut Current Genetics Springer-Verlag 18/3(1990-10-01), 239-243 0172-8083 18:3<239 1990 18 294 https://doi.org/10.1007/BF00318387 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318387 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Izuchi Shinichi Department of Botany, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Terachi Tohru Institute for National Land Utilization and Development, Kyoto Sangyo University, 603, Kyoto, Japan aut NATIONALLICENCE 700 1- Sakamoto Masahiro Plant Biotechnology Department, Life Science Laboratory, Mitsui Toatsu Chemicals, Inc, 297, Mobara, Japan aut NATIONALLICENCE 700 1- Mikami Tetsuo Laboratory of Industrial Crops, Faculty of Agriculture, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Sugita Mamoru Center for Gene Research, Nagoya University, 464-01, Nagoya, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 239-243 0172-8083 18:3<239 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer