caa a22 4500 465757723 CHVBK 20180323111851.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00318386 doi (NATIONALLICENCE)springer-10.1007/BF00318386 Evidence for viral and naked double-stranded RNAs in the basidiomycete Agrocybe aegerita [Elektronische Daten] [Gérard Barroso, Jacques Labarère] Summary: Double-stranded (ds) RNAs were purified from the vegetative mycelium of the commercial basidiomycete Agrocybe aegerita, by gel-filtration chromatography, and their dsRNA nature was demonstrated. They were shown to be distributed in two different RNase-resistant complexes which were separated by sucrose density gradient ultracentrifugation: the first complex is the encapsidated genome (6200 bp) of an isometric mycovirus; the second consists of three naked dsRNA molecules (1900-1800-1700 bp respectively) associated with large vesicles or mitochondria. This is the first report of the presence of naked dsRNA, unencapsidated, viral genome fragments which have co-evolved with their host, or else represent new replicating genetic elements (dsRNA "plasmids”) of unknown function, in a non pathogenic higher fungus. Springer-Verlag, 1990 Agrocybe aegerita nationallicence Mycovirus nationallicence dsRNAs nationallicence Fungal vesicles nationallicence Barroso Gérard Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université de Bordeaux II-INRA, CRA de Bordeaux, BP 81, F-33883, Villenave d'Ornon Dédex, France aut Labarère Jacques Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université de Bordeaux II-INRA, CRA de Bordeaux, BP 81, F-33883, Villenave d'Ornon Dédex, France aut Current Genetics Springer-Verlag 18/3(1990-10-01), 231-237 0172-8083 18:3<231 1990 18 294 https://doi.org/10.1007/BF00318386 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318386 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barroso Gérard Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université de Bordeaux II-INRA, CRA de Bordeaux, BP 81, F-33883, Villenave d'Ornon Dédex, France aut NATIONALLICENCE 700 1- Labarère Jacques Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université de Bordeaux II-INRA, CRA de Bordeaux, BP 81, F-33883, Villenave d'Ornon Dédex, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/3(1990-10-01), 231-237 0172-8083 18:3<231 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer