caa a22 4500 465757758 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318212 doi (NATIONALLICENCE)springer-10.1007/BF00318212 Novel sterile mutants of the fission yeast Schizosaccharomyces pombe which are defective in their response to starvation [Elektronische Daten] [Kenji Kitamura, Tomoko Nakagawa, Chikashi Shimoda] Summary: The mating reaction in the fission yeast, Schizosaccharomyces pombe, is induced by nitrogen deprivation. In order to understand the genetic control of cellular responses to starvation, sterile mutants defective in the mating reaction were isolated and analyzed. Tetrad analysis and complementation tests of ethyl methanesulfonate-induced Ste- mutants revealed three novel ste loci, designated ste11, ste12 and ste13. These genes were mapped on chromosome II. ste11 was allelic to stex; ste12 was tightly linked to ste2 (0.8 cM). Homozygous diploid strains harboring either of these ste alleles were defective in meiosis. Vegetative cells of the ste11 mutant showed the normal resting state in stationary phase. In contrast, the ste12 and ste13 mutant cells rapidly lost viability after nutrient deprivation and the latter were sensitive to heat shock in stationary phase. The transcript level of the mat1-Pi and mei2 genes was greatly reduced in these mutants. These results indicate that the ste12 and ste13 genes are responsible not only for entry into sexual reproduction but also for entry into the G0 phase. Springer-Verlag, 1990 G0 phase nationallicence Mating nationallicence Schizosaccharomyces pombe nationallicence Starvation nationallicence Kitamura Kenji Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut Nakagawa Tomoko Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut Shimoda Chikashi Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut Current Genetics Springer-Verlag 18/4(1990-11-01), 315-321 0172-8083 18:4<315 1990 18 294 https://doi.org/10.1007/BF00318212 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318212 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitamura Kenji Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut NATIONALLICENCE 700 1- Nakagawa Tomoko Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut NATIONALLICENCE 700 1- Shimoda Chikashi Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 315-321 0172-8083 18:4<315 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer