caa a22 4500 465757820 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318208 doi (NATIONALLICENCE)springer-10.1007/BF00318208 Functional analysis of the yeast genome: use of two-dimensional gel electrophoresis to detect genes in randomly cloned DNA sequences [Elektronische Daten] [D. Thoraval, M. Régnacq, P. Neuville, H. Boucherie] Summary: Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel electrophoresis, of the proteins from yeast cells transformed with a yeast genomic DNA library constructed in a multicopy vector. We demonstrate here the applicability of this method for exploring the yeast genome. In addition, we report results which suggest that this method may also be useful for detecting regulatory genes. Springer-Verlag, 1990 S. cerevisiae nationallicence 2-D gel electrophoresis nationallicence Genome analysis nationallicence Thoraval D. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut Régnacq M. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut Neuville P. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut Boucherie H. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut Current Genetics Springer-Verlag 18/4(1990-11-01), 281-286 0172-8083 18:4<281 1990 18 294 https://doi.org/10.1007/BF00318208 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318208 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thoraval D. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut NATIONALLICENCE 700 1- Régnacq M. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut NATIONALLICENCE 700 1- Neuville P. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut NATIONALLICENCE 700 1- Boucherie H. Laboratoire de Génétique, UA CNRS 542, Avenue des Facultés, F-33405, Talence, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 281-286 0172-8083 18:4<281 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer