caa a22 4500 465757839 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318215 doi (NATIONALLICENCE)springer-10.1007/BF00318215 Structure and expression of a single actin gene in Volvox carteri [Elektronische Daten] [Bronislava Cresnar, Wolfgang Mages, Kurt Müller, J. Salbaum, Rüdiger Schmitt] Summary: Southern blot analysis of Volvox carteri DNA indicated the presence of a single actin gene; the nucleotide sequence of that gene is reported here. In comparison with plant animal and fungal actins, the derived primary structure of 377 amino acids is highly conserved yielding similarity values of 79% to 94% (including nonidentical, conservative exchanges). In contrast, the intron structure of the gene is highly unusual: in addition to one intron in the 5′ untranslated region (ten nucleotides upstream of the initiator ATG), it has eight introns in the coding region, only three of which are in locations where introns have previously been reported. Transcription starts 26 nucleotides downstream of the putative TATA box and 70 nucleotides downstream of a conspicuous CCAAT motif. A potential polyadenylation signal, TGTAA, is located 366 nucleotides downstream of the terminator TAA. Northern hybridization indicates that the actin gene is transcribed throughout the Volvox life cycle with only a slight depression during the release of juveniles from mother spheroids. This pattern of gene expression suggests that actin may assume various functional roles in the differentiation and growth of Volvox. Springer-Verlag, 1990 Volvox carteri nationallicence Actin gene nationallicence Introns nationallicence Expression nationallicence Cresnar Bronislava Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut Mages Wolfgang Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut Müller Kurt Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut Salbaum J. Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut Schmitt Rüdiger Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut Current Genetics Springer-Verlag 18/4(1990-11-01), 337-346 0172-8083 18:4<337 1990 18 294 https://doi.org/10.1007/BF00318215 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318215 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cresnar Bronislava Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut NATIONALLICENCE 700 1- Mages Wolfgang Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut NATIONALLICENCE 700 1- Müller Kurt Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut NATIONALLICENCE 700 1- Salbaum J. Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut NATIONALLICENCE 700 1- Schmitt Rüdiger Lehrstuhl für Genetik, Universität Regensburg, W-8400, Regensburg, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 337-346 0172-8083 18:4<337 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer