caa a22 4500 465757847 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318209 doi (NATIONALLICENCE)springer-10.1007/BF00318209 Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae [Elektronische Daten] [Michèle Minet, François Lacroute] Summary: A HeLa cell cDNA library on a yeast expression vector was used to complement auxotrophic markers of Saccharomyces cerevisiae. Clones complementing the ade2-101 mutation harbor a 1.5 kb poly(A)+ tailed insert with a 425 amino acid open reading frame hybridizing with two human mRNAs of 1.5 kb and 3.1 kb. Its 5′ half is homologous to Bacillus subtilis SAICAR synthetase (E.C.6.3.2.6.) and its 3′ terminal half corresponds to the catalytic subunit of Escherichia coli and B. subtilis AIR carboxylase (E.C.4.1.1.21). In agreement with these homologies, pADE2H1 clones complement both ade1 and ade2 mutants of S. cerevisiae, as was also recently reported for a 3.1 kb cDNA isolated from human hepatocytes. Springer-Verlag, 1990 Purine biosynthesis nationallicence Yeasts nationallicence HeLa nationallicence cDNA library nationallicence Minet Michèle Centre de Génétique Moléculaire, C.N.R.S., F-91190, Gif sur Yvette, France aut Lacroute François Centre de Génétique Moléculaire, C.N.R.S., F-91190, Gif sur Yvette, France aut Current Genetics Springer-Verlag 18/4(1990-11-01), 287-291 0172-8083 18:4<287 1990 18 294 https://doi.org/10.1007/BF00318209 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318209 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minet Michèle Centre de Génétique Moléculaire, C.N.R.S., F-91190, Gif sur Yvette, France aut NATIONALLICENCE 700 1- Lacroute François Centre de Génétique Moléculaire, C.N.R.S., F-91190, Gif sur Yvette, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 287-291 0172-8083 18:4<287 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer