caa a22 4500 465757855 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318216 doi (NATIONALLICENCE)springer-10.1007/BF00318216 The β -tubulin gene of Epichloë typhina from perennial ryegrass ( Lolium perenne ) [Elektronische Daten] [Alfred Byrd, Christopher Schardl, Peeranan Songlin, Kim Mogen, Malcolm Siegel] Summary: Epichloë typhina is a biotrophic fungal pathogen which causes choke disease of pooid grasses. The anamorphic state, Acremonium typhinum, is placed in the section Albo-lanosa along with related, mutualistic, seeddisseminated endophytes. As an initial study of gene structure and evolution in Epichloë and related endophytes, the β-tubulin gene, tub2, of the perennial ryegrass choke pathogen (EtPRG) was cloned and sequenced. The coding sequence and the predicted β-tubulin amino acid sequence were highly homologous to the Neurospora crassa homologs, and to one of the two β-tubulin genes of Emericella nidulans. However, two introns characteristic of the N. crassa and Em. nidulans genes were absent in the E. typhina gene. Furthermore, one of the remaining introns possessed the uncommon 5′ splice junction, GC. In contrast to published observations concerning other Ascomycetes, a mutant of EtPRG, selected for resistance to methyl-2-benzimidazole carbamate (benomyl), possessed no alteration of its β-tubulin coding sequence. Springer-Verlag, 1990 β -tubulin nationallicence Epichloë typhina (Acremonium typhinum) nationallicence Clavicipitaceae nationallicence Grass endophyte nationallicence Byrd Alfred Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut Schardl Christopher Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut Songlin Peeranan Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut Mogen Kim Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut Siegel Malcolm Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut Current Genetics Springer-Verlag 18/4(1990-11-01), 347-354 0172-8083 18:4<347 1990 18 294 https://doi.org/10.1007/BF00318216 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318216 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Byrd Alfred Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Schardl Christopher Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Songlin Peeranan Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Mogen Kim Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Siegel Malcolm Department of Plant Pathology, S-305 Agriculture Science Center-North, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 347-354 0172-8083 18:4<347 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer