caa a22 4500 465757871 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00318221 doi (NATIONALLICENCE)springer-10.1007/BF00318221 Electrophoretic karyotyping without the need for generating protoplasts [Elektronische Daten] [K. McCluskey, B. Russell, D. Mills] Summary: Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agarsolidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes. Springer-Verlag, 1990 PFGE nationallicence Phytopathogenic fungi nationallicence Molecular karyotype nationallicence Intact cells nationallicence McCluskey K. Department of Botany and Plant Pathology, Oregon State University, 97331-2902, Corvallis, OR, USA aut Russell B. Genetics Program, Oregon State University, 97331-2902, Corvallis, OR, USA aut Mills D. Department of Botany and Plant Pathology, Oregon State University, 97331-2902, Corvallis, OR, USA aut Current Genetics Springer-Verlag 18/4(1990-11-01), 385-386 0172-8083 18:4<385 1990 18 294 https://doi.org/10.1007/BF00318221 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00318221 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCluskey K. Department of Botany and Plant Pathology, Oregon State University, 97331-2902, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Russell B. Genetics Program, Oregon State University, 97331-2902, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Mills D. Department of Botany and Plant Pathology, Oregon State University, 97331-2902, Corvallis, OR, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/4(1990-11-01), 385-386 0172-8083 18:4<385 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer