caa a22 4500 465757898 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309909 doi (NATIONALLICENCE)springer-10.1007/BF00309909 The DNA-binding protein RAP1 is required for efficient transcriptional activation of the yeast PYK glycolytic gene [Elektronische Daten] [J. McNeil, P. Dykshoorn, J. Huy, S. Small] Summary: We show by deletion mutagenesis, followed by in vivo and in vitro analysis, that the binding of a protein factor to the upstream activation sequence (USA) of the Saccharomyces cerevisiae glycolytic gene PYK, encoding pyruvate kinase, is required for efficient transcription of the corresponding coding region. In addition, gel electrophoretic mobility shift and DNase I protection studies, involving yeast gene products expressed in E. coli, suggest that this trans-acting DNA-binding protein is encoding by the RAP1 gene. The identification of RAP1 binding sites located within the UAS element of the yeast PYK, PGK (phosphoglycerate kinase) and ENO1 (enolase) genes, and in the 5′-upstream region of the ADHI (alcohol dehydrogenase) gene, suggests that a mechanism of coordinate gene expression involving several of the glycolytic genes may exist in yeast. Springer-Verlag, 1990 Yeast nationallicence Trans-acting Factor nationallicence RAP1 nationallicence McNeil J. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Dykshoorn P. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Huy J. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Small S. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut Current Genetics Springer-Verlag 18/5(1990-12-01), 405-412 0172-8083 18:5<405 1990 18 294 https://doi.org/10.1007/BF00309909 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309909 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McNeil J. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Dykshoorn P. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Huy J. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Small S. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 405-412 0172-8083 18:5<405 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer