caa a22 4500 465757901 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309911 doi (NATIONALLICENCE)springer-10.1007/BF00309911 CBP 1 function is required for stability of a hybrid cob-oli 1 transcript in yeast mitochondria [Elektronische Daten] [Telsa Mittelmeier, Carol Dieckmann] Summary: The nuclear gene product CBP1 stabilizes cytochrome b transcripts in yeast mitochondria. In cbp1 mutant strains, cytochrome b gene (cob) transcripts are not detectable by Northern blot analysis. The results of previous studies led to the hypothesis that CBP1 interacts with the 5′-untranslated sequence of the cob mRNA, or pre-mRNA, to stabilize the message. To determine what portion of the cob leader is sufficient for interaction with CBP1, we have investigated the stability of transcripts from a novel hybrid gene, cob-oli1, in which the 5′-terminal third of the cob leader sequence was fused to the coding sequence of the gene for ATP synthase subunit 9, oli1. The hybrid cob-oli1 transcript was stale in a strain wild-type at the CBP1 locus, but was undetectable in the cbp1 mutant background. That the cob-oli1 transcript was translated to produce ATP synthase subunit 9 in CBP1 strains containing the cob-oli1 gene was verified by 35S-methionine labeling of mitochondrial proteins. We conclude that the 5′-terminal portion of the cob message is sufficient for CBP1 function and discuss the hypothesis that CBP1 interacts directly with this region of the transcript to promote cob mRNA stability. Springer-Verlag, 1990 Mitochondria nationallicence CBP 1 nationallicence RNA stability nationallicence Mittelmeier Telsa Department of Molecular and Cellular Biology, University of Arizona, Life Sciences South 426, 85721, Tucson, AZ, USA aut Dieckmann Carol Department of Molecular and Cellular Biology, University of Arizona, Life Sciences South 426, 85721, Tucson, AZ, USA aut Current Genetics Springer-Verlag 18/5(1990-12-01), 421-428 0172-8083 18:5<421 1990 18 294 https://doi.org/10.1007/BF00309911 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309911 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mittelmeier Telsa Department of Molecular and Cellular Biology, University of Arizona, Life Sciences South 426, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Dieckmann Carol Department of Molecular and Cellular Biology, University of Arizona, Life Sciences South 426, 85721, Tucson, AZ, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 421-428 0172-8083 18:5<421 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer