caa a22 4500 46575791X CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309915 doi (NATIONALLICENCE)springer-10.1007/BF00309915 Cloning of the Trichoderma reesei pyr G gene and its use as a homologous marker for a high-frequency transformation system [Elektronische Daten] [Franz Gruber, Jaap Visser, Christian Kubicek, Leo de Graaff] Summary: The Trichoderma reesei orotidine-5′-phosphate decarboxylase gene was isolated by heterologous hybridization with the corresponding Neurospora gene as a probe. A 2.7 kb SalI fragment, which exclusively hybridized to the Neurospora gene, was subcloned in pGEM-5Zf(+). This subclone was termed pFG1 and was used to transform a Trichoderma reesei pyrG- negative mutant to PYR+. The transformation frequency in this homologous system was up to 12000 transformants per μg DNA. About one-fifth of the transformants tested were abortive. Perfect mitotic stability was found in half of the non-abortive transformants, correlating with vector integration at homologous and ectopic loci. In the unstable transformants the transforming DNA appears to be present in the form of extrachromosomal elements. Springer-Verlag, 1990 Trichoderma reesei nationallicence Homologous transformation nationallicence pyr G nationallicence Vector integration nationallicence Gruber Franz Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut Visser Jaap Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 BM, Wageningen, The Netherlands aut Kubicek Christian Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut de Graaff Leo Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 BM, Wageningen, The Netherlands aut Current Genetics Springer-Verlag 18/5(1990-12-01), 447-451 0172-8083 18:5<447 1990 18 294 https://doi.org/10.1007/BF00309915 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309915 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gruber Franz Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut NATIONALLICENCE 700 1- Visser Jaap Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 BM, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kubicek Christian Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060, Wien, Austria aut NATIONALLICENCE 700 1- de Graaff Leo Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 BM, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 447-451 0172-8083 18:5<447 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer