caa a22 4500 465757944 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309919 doi (NATIONALLICENCE)springer-10.1007/BF00309919 Expression of the wheat chloroplast gene for CF0 subunit IV of ATP synthase [Elektronische Daten] [Anna-Stina Höglund, Aine Plant, John Gray] Summary: The nucleotide sequence of the wheat chloroplast atp I gene encoding CF0 subunit IV of ATP synthase has been determined. The gene encodes a polypeptide of 247 amino acid residues with high sequence similarity to subunit IV from other plant chloroplasts and from cyanobacteria. The polypeptide shows sequence homology to the C-terminus of the F0 a subunit of Escherichia coli ATP synthase and subunit 6 of mitochondrial ATP synthase. The atp I gene is co-transcribed with the atp H, atp F and atp A genes for other subunits of ATP synthase in wheat. A gene-fusion of most of the atpI coding region with cro 1-lacI'-lacZ' has been constructed in pEX2 and the fusion-protein has been used to raise antibodies in rabbits. The antibodies react with a polypeptide of 17 kDa in wheat thylakoid membranes indicating that the wheat atpI gene is expressed at the protein level. A model for the organisation of the polypeptide in the thylakoid membrane with four membrane-spanning segments is proposed. Springer-Verlag, 1990 H+-ATPase nationallicence Gene-fusion nationallicence Thylakoid membrane nationallicence Höglund Anna-Stina Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut Plant Aine Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut Gray John Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut Current Genetics Springer-Verlag 18/5(1990-12-01), 471-476 0172-8083 18:5<471 1990 18 294 https://doi.org/10.1007/BF00309919 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309919 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Höglund Anna-Stina Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut NATIONALLICENCE 700 1- Plant Aine Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut NATIONALLICENCE 700 1- Gray John Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 471-476 0172-8083 18:5<471 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer