caa a22 4500 465757952 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309914 doi (NATIONALLICENCE)springer-10.1007/BF00309914 Fincham J. Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, Cambridge, England, UK aut Generation of new functional mutant alleles by premeiotic disruption of the Neurospora crassa am gene [Elektronische Daten] [J. Fincham] Summary: In the further analysis of a cross in which the mis-sense allele, am 3, of the Neurospora crassa am (glutamate dehydrogenase) gene was present in one parent together with two ectopic wild-type gene copies, one ascus was identified in which the two ectopic copies had been inactivated by the RIP process whereas the am 3 allele continued to produce its characteristic enzyme variety in active, but heat-sensitive, form. The am 3 allele had also acquired a new HindIII restriction site. It had no detectable methylation. The mutations responsible respectively for the new restriction site and the modified enzyme properties were separated from each other, and from the original am 3 mutation, by selecting for intragenic recombination on either side of the am 3 site. In this way two new effectively wild-type alleles were generated, one characterised by its heat-sensitive and kinetically modified enzyme product and the other by a new HindIII site. These results demonstrate that the RIP phenomenon can be a source of new functional alleles. Springer-Verlag, 1990 Neurospora crassa nationallicence Mutation nationallicence RIP phenomenon nationallicence Current Genetics Springer-Verlag 18/5(1990-12-01), 441-445 0172-8083 18:5<441 1990 18 294 https://doi.org/10.1007/BF00309914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309914 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Fincham J. Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, Cambridge, England, UK aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 441-445 0172-8083 18:5<441 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer