caa a22 4500 465757979 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309907 doi (NATIONALLICENCE)springer-10.1007/BF00309907 Fujimura Hiro-aki Laboratory for Molecular Biology, Pharma Research Laboratories, Hoechst Japan Ltd., 1-3-2 Minamidai, 350, Kawagoe, Japan aut Molecular cloning of the DAC2/FUS3 gene essential for pheromone-induced G1-arrest of the cell cycle in Saccharomyces cerevisiae [Elektronische Daten] [Hiro-aki Fujimura] Summary: Mating pheromones, known as a and α-factors, arrest the division of cells of opposite mating types, α and a respectively, in Saccharomyces cerevisiae. I have cloned the DAC2 gene, which is required for both pheromone-induced division-arrest and cell-fusion during conjugation. The constructed dac2::LEU2 null mutation leads to defects in both pheromone-induced divisionarrest and cell-fusion during conjugation; it also suppresses the growth defect caused by the gpal mutation (a mutation in the α subunit of the S. cerevisiae G protein). These results indicate that DAC2 may be the same gene as FUS3, which was recently isolated by Elion et al. (1990) as a gene essential for cell-fusion during conjugation. The dac2::LEU2 null mutant also showed morphological alterations in response to mating pheromones. I show here that the DAC2 product plays an essential role in both the division-arrest signalling pathway of the yeast pheromone response and in cell-fusion during conjugation. Springer-Verlag, 1990 Pheromone response pathway nationallicence Signal transduction nationallicence Cell cycle control nationallicence Yeast DAC2/FUS3 gene nationallicence Current Genetics Springer-Verlag 18/5(1990-12-01), 395-400 0172-8083 18:5<395 1990 18 294 https://doi.org/10.1007/BF00309907 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309907 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Fujimura Hiro-aki Laboratory for Molecular Biology, Pharma Research Laboratories, Hoechst Japan Ltd., 1-3-2 Minamidai, 350, Kawagoe, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 395-400 0172-8083 18:5<395 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer