caa a22 4500 465757987 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309916 doi (NATIONALLICENCE)springer-10.1007/BF00309916 Transformation of Trichoderma viride using the Neurospora crassa pyr 4 gene and its use in the expression of a Taka-amylase A gene from Aspergillus oryzae [Elektronische Daten] [Cheng Cheng, Norihiro Tsukagoshi, Shigezo Udaka] Summary: A pyrG mutant of Trichoderma viride, a very efficient cellulase producer, was isolated from among 5-fluoroorotic acid-resistant mutants. The mutation was complemented with the pyr4 gene of Neurospora crassa and used as a selection marker for the transformation of T. viride. A plasmid vector, pDJB1-Taa, carrying both the pyr4 gene and a gene encoding Taka-amylase A from Aspergillus oryzae, was constructed and introduced into protoplasts of T. viride pyrG-. The transformation frequency was 1-10 transformants (3 on average) per μg DNA. One transformant showed highly elevated α-amylase production (about 17 times higher than the recipient level) and the integration of more than one copy of the Taka-amylase gene. Springer-Verlag, 1990 Transformation of Trichoderma viride nationallicence pyr 4 gene of Neurospora crassa nationallicence Expression of the Taka-amylase A gene nationallicence Cheng Cheng Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut Tsukagoshi Norihiro Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut Udaka Shigezo Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut Current Genetics Springer-Verlag 18/5(1990-12-01), 453-456 0172-8083 18:5<453 1990 18 294 https://doi.org/10.1007/BF00309916 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309916 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Cheng Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut NATIONALLICENCE 700 1- Tsukagoshi Norihiro Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut NATIONALLICENCE 700 1- Udaka Shigezo Faculty of Agriculture, Nagoya University, Chikusa, 464, Nagoya, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 453-456 0172-8083 18:5<453 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer