caa a22 4500 465758002 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00309910 doi (NATIONALLICENCE)springer-10.1007/BF00309910 Two substitutions at the same position in the mitochondrial cytochrome b gene of S. cerevisiae induce a mitochondrial myxothiazol resistance and impair the respiratory growth of the mutated strains abbeit maintaining a good electron transfer activity [Elektronische Daten] [Thierry Tron, Danielle Lemesle-Meunier] Summary: Two cytochrome b respiratory—deficient mutants were sequenced and their DNA base change identified, leading to the replacement of glycine (G137 by valine or glutamicacid. No variation in their cytochrome b content with regard to cytochrome oxidase and cytochrome (c+c1) was found to have occurred. Their cellular respiratory activity with various substrates was partly conserved and was totally inhibited by antimycin A. Their ubiquinol (QH2)-cytochrome c reductase/mole cytochrome b activity decreased by about 50%. Paradoxically their growth on respiratory substrate was abolished. Both mutants retained a high-affinity binding site for antimycin A, and exhibited a myxothiazol—resistance at the mitochondrial level. It seems likely that the mutated position (137), which belongs to the ubiquinol oxidizing domain of the bc1 complex, interferes, directly or indirectly, with the respiratory growth capacity of the cell. Springer-Verlag, 1990 Mitochondrial cytochrome b split gene nationallicence Mutational alteration nationallicence DNA sequence analysis nationallicence Myxothiazol resistance nationallicence Oxidative phosphorylation nationallicence Tron Thierry Unité de Métabolisme Énergétique, L.C.B., B.P. no3, F-13275, Marseille Cedex 9, France aut Lemesle-Meunier Danielle Unité de Métabolisme Énergétique, L.C.B., B.P. no3, F-13275, Marseille Cedex 9, France aut Current Genetics Springer-Verlag 18/5(1990-12-01), 413-419 0172-8083 18:5<413 1990 18 294 https://doi.org/10.1007/BF00309910 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00309910 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tron Thierry Unité de Métabolisme Énergétique, L.C.B., B.P. no3, F-13275, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Lemesle-Meunier Danielle Unité de Métabolisme Énergétique, L.C.B., B.P. no3, F-13275, Marseille Cedex 9, France aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/5(1990-12-01), 413-419 0172-8083 18:5<413 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer