caa a22 4500 46575807X CHVBK 20180323111852.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312600 doi (NATIONALLICENCE)springer-10.1007/BF00312600 Restriction map of the mitochondrial DNA of the true slime mould, Physarum polycephalum [Elektronische Daten] linear form and long tandem duplication [H. Takano, S. Kawano, Y. Suyama, T. Kuroiwa] Summary: The mitochondrial DNA (mtDNA) of the true slime mould, Physarum polycephalum strain CH934xCH938, was isolated and characterized by restriction mapping. Cloned fragments of the mtDNA were assembled and used to construct the restriction map. This map showed that the mtDNA was a linear molecule of 86.0 kb with a tandem duplication of 19.6 kb. The terminal fragments were identified by sensitivity to Bal31 exonuclease. One of the duplications was located at the right end and the other was located 5 kb from the left end. Each duplicated segment contained 26 restriction sites for ten enzymes and these restriction sites were completely conserved in each duplication. Genes for the large and small rRNAs were mapped to positions about 30 kb from the right end of the mtDNA by hybridization with its own rRNAs. With the exception of a probe for the gene for the large rRNA in Tetrahymena pyriformis mtDNA, various probes from the mtDNAs of Saccharomyces cerevisiae and T. pyriformis showed no significant hybridization to any of the restriction fragments of the mtDNA from P. polycephalum. Springer-Verlag, 1990 Physarum nationallicence mtDNA nationallicence Restriction map nationallicence Takano H. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut Kawano S. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut Suyama Y. Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA aut Kuroiwa T. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut Current Genetics Springer-Verlag 18/2(1990-08-01), 125-131 0172-8083 18:2<125 1990 18 294 https://doi.org/10.1007/BF00312600 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312600 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takano H. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawano S. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut NATIONALLICENCE 700 1- Suyama Y. Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Kuroiwa T. Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 125-131 0172-8083 18:2<125 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer