caa a22 4500 465758096 CHVBK 20180323111852.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312604 doi (NATIONALLICENCE)springer-10.1007/BF00312604 Cloning and expression of a second Aspergillus niger pectin lyase gene ( pel A): Indications of a pectin lyase gene family in A. niger [Elektronische Daten] [J. Harmsen, M. Kusters-van Someren, J. Visser] Summary: Using the previously cloned Aspergillus niger N756 pectin lyase D gene as a probe, the corresponding pelD gene has been isolated from a genomic library of the loboratory strain A. niger N400. This gene encodes PLD, previously described as PLI, which is one of the two major pectin lyases isolated from the commeriial pectinase preparation Ultrazym®. Heterologous hybridization of the A. niger N400 genomic library with the pelD gene led to the isolation of another five genes: pelA, B, C, E, and F. These genes differ in their hybridization patterns with probes containing either the entire pelD gene, or 5′ or 3′ parts thereof. By partial sequencing, and expression in an A. niger transformant containing multiple copies of the pelA gene, we show that this gene, which hybridizes strongest with the pelD gene, encodes the other major pectin lyase from Ultrazym®, PLII. Springer-Verlag, 1990 Aspergillus niger nationallicence pel genes nationallicence Pectin lyase nationallicence Heterologous hybridization nationallicence Harmsen J. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Kusters-van Someren M. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Visser J. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Current Genetics Springer-Verlag 18/2(1990-08-01), 161-166 0172-8083 18:2<161 1990 18 294 https://doi.org/10.1007/BF00312604 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312604 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harmsen J. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kusters-van Someren M. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Visser J. Department of Genetics, Section Molecular Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 161-166 0172-8083 18:2<161 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer