caa a22 4500 465758118 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312597 doi (NATIONALLICENCE)springer-10.1007/BF00312597 A sequence that directs transcriptional initiation in yeast [Elektronische Daten] [A. Healy, R. Zitomer] Summary: While RNA polymerase II of the yeast Saccharomyces cerevisiae initiates transcription at discrete sites, these sites are located over a wide range of distances from the TATA box for different genes. This variability has led to a number of proposals for consensus sequences located at the initiation site which, in conjunction with the TATA box, would direct initiation. We tested this hypothesis via oligonucleotide-directed mutagenesis, by placing the sequence CAAG, a member of one of these consensus sequences, upstream of the coding sequence of the CYC7 gene at a site at which initiation does not occur. The distance between the TATA sequence and this putative initiation site was varied by inserting it into the wild-type gene and three deletion mutants. The results demonstrated that this sequence can serve as an initiation site when located 49, 77, or 106 bp from the TATA sequence, but not when located 30 bp away. Springer-Verlag, 1990 CYC7 gene nationallicence Cytochrome c nationallicence TATA box nationallicence Healy A. Department of Biological Sciences, State University of New York at Albany, 12222, Albany, NY, USA aut Zitomer R. Department of Biological Sciences, State University of New York at Albany, 12222, Albany, NY, USA aut Current Genetics Springer-Verlag 18/2(1990-08-01), 105-109 0172-8083 18:2<105 1990 18 294 https://doi.org/10.1007/BF00312597 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312597 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Healy A. Department of Biological Sciences, State University of New York at Albany, 12222, Albany, NY, USA aut NATIONALLICENCE 700 1- Zitomer R. Department of Biological Sciences, State University of New York at Albany, 12222, Albany, NY, USA aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 105-109 0172-8083 18:2<105 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer