caa a22 4500 465758142 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312605 doi (NATIONALLICENCE)springer-10.1007/BF00312605 Direct evidence that restriction endonucleases may under estimate the degree of divergence between molecules [Elektronische Daten] [C. De Giorgi, F. De Luca, C. Stefanile, G. Pesole, C. Saccone] Summary: We studied two polymorphic forms of mtDNA extracted from A. lixula eggs. In order to compare and to quantitate the variability, we sequenced specific regions of the two molecules. In this way, we obtained a precise measurement of the variability within two haplotypes. We also obtained a direct demonstration that some differences in nucleotide sequence can escape detection when restriction endonuclease analysis is used. Our results underline the unreliability of the use of restriction mapping to estimate divergence between relatively short and closely related DNA sequences. Springer-Verlag, 1990 Intraspecific mtDNA divergence nationallicence Endonucleases nationallicence Sea urchins nationallicence De Giorgi C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut De Luca F. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut Stefanile C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut Pesole G. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut Saccone C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut Current Genetics Springer-Verlag 18/2(1990-08-01), 167-168 0172-8083 18:2<167 1990 18 294 https://doi.org/10.1007/BF00312605 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312605 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Giorgi C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut NATIONALLICENCE 700 1- De Luca F. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut NATIONALLICENCE 700 1- Stefanile C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut NATIONALLICENCE 700 1- Pesole G. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut NATIONALLICENCE 700 1- Saccone C. Departimento di Biochimica e Biologia Molecolare, Centro di Studio sui Mitocondri e Metabolismo Energetico, (CNR), University of Bari, 1-70100, Bari, Italy aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 167-168 0172-8083 18:2<167 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer