caa a22 4500 465758150 CHVBK 20180323111853.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00312601 doi (NATIONALLICENCE)springer-10.1007/BF00312601 The mitochondrial nad5 gene of sugar beet ( Beta vulgaris ) encoding a subunit of the respiratory NADH dehydrogenase [Elektronische Daten] [W. Ecke, U. Schmitz, G. Michaelis] Summary: We have isolated the nad5 gene from the N-cytoplasm of Beta vulgaris, by heterologous hybridization with a nad5 probe from Chlamydomonas reinhardtii, and have determined the DNA sequence. The gene has a length of 3082 bp and consists of three exons and two introns, 459, 1269, 270, 848 and 357 bp in length, respectively. It has a similarity of 95.1% at the DNA level to the nad5 gene of Oenothera and the two respective proteins show an overall level of amino acid identity of 88.7%. Compared to Oenothera the reading frame of the first exon is extended in the 5′ direction and in the third exon there is a frameshift shortening the reading frame by 96 bp and changing the last 48 codons. The nad5 gene is a single copy gene in the N- and S-cytoplasm of Beta vulgaris and the organisation of the gene shows no apparent differences in the two cytoplasms. The gene is actively expressed; three major transcripts are detectable. The transcript patterns have been compared between the two cytoplasms and between different plant tissues. Springer-Verlag, 1990 Beta vulgaris nationallicence Mitochondrial nad5 gene nationallicence NADH-dehydrogenase nationallicence CMS nationallicence Ecke W. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut Schmitz U. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut Michaelis G. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut Current Genetics Springer-Verlag 18/2(1990-08-01), 133-139 0172-8083 18:2<133 1990 18 294 https://doi.org/10.1007/BF00312601 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00312601 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ecke W. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut NATIONALLICENCE 700 1- Schmitz U. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut NATIONALLICENCE 700 1- Michaelis G. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany aut NATIONALLICENCE 773 0- Current Genetics Springer-Verlag 18/2(1990-08-01), 133-139 0172-8083 18:2<133 1990 18 294 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer